This committee shares successes and addresses challenges in the utilization of various types of cardiac tissue, including but not limited to isolating, culturing, treating, and assessing viable cardiac slices, cardiomyocytes, adipose tissue, coronary artery smooth muscle cells, endothelial cells, fibroblasts, and valve tissue. Our objective is to exchange results and protocols, whether successful or unsuccessful, to enhance our methodologies and improve the impact and translatability of our research. This platform will also serve as an opportunity for members to present new ideas and seek input from peers, fostering innovation and collaboration in our field.